A quantitative reverse transcriptase PCR (qRT-PCR) unveiled that despite the fact that expression of Vim and Gfap was considerably upregulated as the condition progressed, no transcriptional activation of SOD1H46R, Sqstm1 (p62), and Map1lc3a/Map1lc3b (LC3A/LC3B) was noticed (Figure S4)
Related outcomes ended up obtained in samples from the brainstem, cerebellum, and cervical twine, but not from the cerebral cortex (Figure S7). These outcomes suggest that the accumulation of insoluble…