A rating of one is equal to demyelination of one 3rd of the fibers, even though a rating of two is equivalent to demyelination of two thirds of the fibers of the myelin tract. Iba-one+ microglial cells ended up counted manually in a blinded trend
CNS tissues had been lysed in mobile lysis buffer (Cell Signaling), supplemented with Full Protease Inhibitor Cocktail and PhosSTOP Phospatase inhibitors (equally Roche), for 30 min, sonicated and centrifuged at…