Ues were lacZ+ cells (Figs. 2C ). Few lacZ+ cells at the
Ues were lacZ+ cells (Figs. 2C ). Few lacZ+ cells at the urethral plate and anorectal epithelium were observed at e13.5 and e15.5 (Figs. 2C ). In addition, mesenchymal cells…
XRxQx(S/x) (R-2 motif), xRx(Y/x)Q(S/A
XRxQx(S/x) (R-2 motif), xRx(Y/x)Q(S/A/x)x (R-3 motif), or xxQx(S/x) (R-none motif) . The presequence binds to the active site of theMPP in an extended conformation. In addition some presequences can be…
H PBS and fixed with 4 paraformaldehyde for 10 min at room temperature
H PBS and fixed with 4 paraformaldehyde for 10 min at room temperature and then rinsed three times with PBS. Cells were then permeabilized in PBS containing 0.1 Triton X-100…