Uantitative evaluation of protein [(A) CREB; (E) DARPP; (K) ERK; (O) GluA] and phosphoprotein [(B) PCREB; (F) Thr and Thr PDARPP; (L) PERK; (P) PGluA] levels normalized to actin (mean optical density SEM).Protein levels were measured in prenatal saline treated (PSAL) and prenatalcocaine (PCOC) treated mice administered regular saline (sal), cocaine ( mgkg; coc) or D agonist, SKF ( mgkg; skf) as adults.Information are represented as percentage of PSAL mice treated with saline (PSAL sal).p p .PCOC pretreatment groups vs.PSAL pretreatment groups.p p coc or skf treated mice vs.sal treated mice within the same prenatal therapy.Error bars represent SEM.N micegroup.Frontiers in Psychiatry Kid and Neurodevelopmental PsychiatryDecember Volume Report Tropea et al.Altered molecular signaling following prenatal cocaineFIGURE Effect of prenatal cocaine remedy on basal, cocaine and SKF induced protein phosphorylation in the nucleus accumbens of adult mice.Representative immunoblots and quantitative analysis of protein [(A) CREB; (E) DARPP; (K) ERK; (O) GluA] and phosphoprotein [(B) PCREB; (F) Thr and Thr PDARPP; (L) PERK; (P) PGluA] levels normalized to actin (imply optical density SEM).Protein levels had been measured in prenatal saline (PSAL)treated and prenatal cocaine (PCOC) treated mice administered regular saline (sal), cocaine ( mgkg; coc) or D agonist, SKF ( mgkg; skf) as adults.Information are represented as percentage of PSAL mice treated with saline (PSAL sal).p p .PCOC pretreatment groups vs.PSAL pretreatment groups.p p coc or skf treated mice vs.sal treated mice within the identical prenatal remedy.Error bars represent SEM.N micegroup.www.frontiersin.orgDecember Volume Short article PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21563299 Tropea et al.Altered molecular signaling following prenatal cocaine(Figure F) having a trend toward reduced levels of Thr PDARPP (Figure G).Examination of ERK revealed no alter in the basal levels of ERK or PERK in PCOC mice (Figures K,L, respectively).Examination of GluA revealed substantially higher levels of basal GluA and PGluA in PCOC mice compared to PSAL mice (Figures O,P, respectively).Examination of cocaine or SKF induced adjustments in phosphoprotein levels revealed that cocaine or SKF significantly improved PCREB in PSAL and PCOC mice in comparison to saline treated mice (Figure C, PSAL coc vs.PSAL sal and PCOC coc vs.PCOC sal and Figure D, PSAL skf vs.PSAL sal and PCOC skf vs.PCOC sal, respectively).Moreover, the raise in PCREB observed in PCOC mice was considerably augmented in comparison with PSAL mice (Figure C, PCOC coc vs.PSAL coc; Figure D, PCOC skf vs.PSAL skf).Similarly cocaine or SKF Autophagy therapy drastically enhanced Thr PDARPP levels in PSAL and PCOC mice (Figures G,I, respectively) with significantly augmented levels evident in PCOC mice in comparison with that observed in PSAL mice (Figure G, PCOC coc vs.PSAL coc; Figure I, PCOC skf vs.PSAL skf).Cocaine or SKF treatment drastically decreased Thr PDARPP levels in PSAL mice (Figures H,J, respectively).In PCOC mice, cocaine remedy had no effect on Thr PDARPP levels (Figure H) whereas SKF considerably decreased Thr PDARPP levels (Figure J) to levels that have been significantly decrease than that seen in PSAL mice (Figure J, PCOC skf vs.PSAL skf).Examination of PERK levels revealed that cocaine or SKF therapy considerably increased PERK levels in both PSAL and PCOC mice (Figures M,N, respectively) with substantially augmented levels evident in PCOC mice compared to PSAL mice (Figure M, PCOC.