Was found [65]. Yet another function by Rampazzi et al. examined a Neolithic hypogeum web-site situated in Sardinia, decorated with red and black wall paintings. This is Domus de Janas (Residence of your Fairies, or Household with the Witches). Once again, the objective on the operate was to know the pictorial strategy. The result obtained, D-Phenylalanine MedChemExpress analyzing samples from various graves, confirmed the information located within the preceding study, namely the usage of egg, as a binder to lay out the color [66]. Brecoulaki et al. examined Mycenaean wall paintings inside the “Palace of Nestor” in Pylos (Western Messenia, Greece), dated in the Late Bronze Age (ca. 1200 BC), for the goal to establish the initial extensive analytical documentation for the study with the painting methods practiced throughout the Late Bronze Age. The samples studied employing GC-MS and Py-GC-MS showed egg, animal glue and plant gums. The authors believe that, for the very first time, tempera method has been identified for such an early period within the history of ancient Greek mural painting [67]. A study has been carried out around the polychromy of some fragmented architectural reliefs in the Palace of Apries in Memphis, Egypt (26th Dynasty, ca. 58968 BCE The samples taken had been studied using FTIR to determine the class of organic substances and GCMS. Polysaccharides have been located, identified as plant gums, utilised as binding media, in some samples. Other fragments showed animal glue presumably mixed with egg. One more interesting data regarding the presence of synthetic wax (applied through ancient conservation therapies) and as beeswax, around the surface with the paintings [68]. A paper of Colombini et al. shows the analysis of a wide range of raw plant gums, watercolors, and naturally aged reference painting specimens together with the aim to optimize the analytical process, but also to gather data for constructing a information set, which is beneficial forAppl. Sci. 2021, 11,10 ofbinder identification when a chemometric strategy for instance principal element analysis (PCA) is applied. The analytical methodology was applied for the study of wall paintings (4thrd centuries B.C) of Macedonia [69]. A study on the wall paintings with the Abu El Leaf Monastery, also known as Deir Abu Lifa, is considered one of the most important and renowned monasteries in Fayoum Oasis, Egypt, was carried out. Arabic Gum as organic binding medium suggests that tempera strategy was used in the Abu El Leaf Monastery in Fayoum Oases [70]. A study conducted by Guasch-Ferret al. (2019) viewed as samples of Maya wall paintings of ten archaeological internet sites situated in Campeche and Yucatan regions. This work, carried out by GC-MS, defined the presence, in significant amounts, of a series of monosaccharides, getting glucose and mannose involving probably the most abundantly discovered. This detection opens the question of your deliberate employ of those organic polymers as additives to enhance workability and mechanical properties inside the preparation layer mortar and to confer cohesion for the pigments within the paint layer. Regrettably, the compact sample size and Thiacetazone Epigenetics sensitivity of the analytical approach avert the identification of your vegetal gum applied. Identification is additional difficult if it is assumed that the samples from wall paintings are composed of mixtures of polysaccharide components from bark and numerous parts of plants, which is usually quickly extracted in water [71]. Pallecchi et al. conducted an comprehensive study around the pictorial strategy utilized inside the Etruscan tomb “Tomba della Quadriga.