CL to construct phylogenetic trees mainly because these two genes are deemed normal plant DNA barcoding markers with high discriminatory energy amongst angiosperms [22,23]. This study aimed to produce complete genome datasets and use a part of them as a promising showcase to construct the draft in the chloroplast genome and analyze the universal DNA barcode-based genetic relationships for D. aromatica. two. Materials and Methods 2.1. Plant Materials samples of a single silica-gel dried twig and one particular fresh leaf derived from 1 individual healthy D. aromatica seedling (Figure 1) have been collected for later laboratory perform. Silica-gel dried twigs were utilized simply because they allow simpler collection of sawdust having a drill tool than2. Materials and Solutions 2.1. Plant MaterialsForests 2021, 12, 1515 3 of 14 Samples of one silica-gel dried twig and one fresh leaf derived from a single person healthier D. aromatica seedling (Figure 1) had been collected for later laboratory perform. Silica-gel dried twigs had been made use of because they allow a lot easier collection of sawdust having a drill tool than twigs. The The seedling was originated Lingga Island in Riau Archipelago and and fresh fresh twigs.seedling was originated from from Lingga Island in Riau Archipelago has has raised for five years within the the Komatsu-FORDA Conservation nursery, Forest Research beenbeen raised for five years VU0422288 Purity & Documentation inKomatsu-FORDA Conservation nursery, Forest Investigation and and Development Center, Forestry and Environmental Investigation Improvement and InnoDevelopment Center, Forestry and Environmental Research Development and Innovation vation Ministry of Atmosphere and Forestry in Bogor, West Java, Indonesia. Agency,Agency, Ministry of Environment and Forestry in Bogor, West Java, Indonesia.(b)Figure 1. Dryobalanops aromatica samples had been utilised in this study. Silica-dried twig (a); Fresh leaves Figure 1. Dryobalanops aromatica samples were utilised in this study. Silica-dried twig (a); Fresh leaves (b). (b).2.2. Genomic DNA Extraction 2.two. Genomic DNA Extraction Genomic DNA was extracted by using the modified cetyl trimethyl ammonium bromide (CTAB) process [24]. The CTAB buffer contains 1 PVP, five M NaCl, 0.5 M EDTA, Genomic DNA was extracted by utilizing the modified cetyl trimethyl ammonium bro10 CTAB remedy, and dHThe D. aromatica fresh leaf was PVP, 5 with CTAB buffer and mide (CTAB) technique [24]. 2 O. CTAB buffer consists of 1 mixed M NaCl, 0.five M EDTA, ground manually applying a dH2O. D. aromatica beforeleaf was mixed the extraction process. 10 CTAB answer, and mortar and pestle fresh proceeding to with CTAB buffer and Meanwhile, the twig was mortar andobtain fine sawdust employing athe extraction course of action. ground manually applying a drilled to pestle prior to proceeding to Dremel 3000 Rotary Tool prior to being mixed with CTAB buffer and mashed with Dremel 3000 Rotary Tool Meanwhile, the twig was drilled to obtain fine sawdust employing a a mortar and pestle for additional becoming mixed withThe high quality ofand mashed with a mortar and pestle for further before DNA extraction. CTAB buffer genomic DNA was evaluated using agarose gel electrophoresis performed by Mupid exU,DNAthe purity of genomic DNA was assessed DNA extraction. The high-quality of genomic and was evaluated applying agarose gel electrousing sn-Glycerol 3-phosphate Epigenetic Reader Domain Nanophotometer Mupid exU, and Thepurity of genomic DNA was assessed using phoresis performed by IMPLEN NP80. the A260/280 ratio of 1.8 is encouraged for sequencing. DNAIMPLEN NP80. The A260/280 a Qubit 1.eight is suggested for sequencNanophotometer quantity was measure.