Y, 16 h in migration assay, 8 h in tube formation assay and 12 and 24 h in qRT-PCR. Final results: ADSC-EVs group showed pretty much 1 point 5 to twice improve of proliferation, migration and tube formation function when compared with PBS group. Furthermore, gene expressions for lymphatic markers including VEGFR-3, Lyve-1, Podoplanin, Prox-1 were also shown almost two to five occasions improve inside the ADSC-EVs group. Summary/Conclusion: The present study showed lymphangiogenic effects of EVs derived from ADSCs, which result in new therapy selections for chronic lymphedema. Additional research are needed to elucidate what kind of molecular in ADSC-EVs operates in LEC. In vivo research applying mouse lymphedema model are also needed to confirm the biological function of ADSCEVs. EVs for cell no cost therapy are much less prospective danger in comparison with stem cell transplantation and may very well be promising tool for patients affected by lymphedema. Funding: JSPS Kakenhi; Takeda Science Foundation.PT12.Embryonic stem cell-derived extracellular vesicle-mimetic nanovesicles rescue erectile function by enhancing penile neurovascular regeneration within the streptozotocin-induced diabetic mouse Kang-Moon Songa, Mi-Hye Kwona, Guonan Yina, Kalyan Ghataka, Nguyen Nhat Minha, Min Ji Choia, Jiyeon Ocka, Yong Song Ghob, Ji-Kan Ryua and Jun-Kyu Suhaa National Study Center for Sexual BTLA/CD272 Proteins custom synthesis Medicine and Department of Urology, Inha University School of Medicine, incheon, Republic of Korea; b Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of KoreaJichi Medical Unversity, Tochigi, Japan; bDepartment of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Health-related University, Shinjyuku-ku, JapanIntroduction: Lymphedema is chronic oedema of limbs caused by the accumulation of lymphatic fluid and characterized by a progressive disorder of the smooth muscle cells in the lymphatic channels. Transplantation of adipose-derived mesenchymal stem cells (ADSCs) has been reported to improve the severity of lymphedema, nonetheless, the detailed mechanism has not been elucidated yet. Extracellular vesicles(EVs) derived from mesenchymal stem cells have already been reported to have functions which include cancer improvement, angiogenesis, suppression of inflammation, regeneration of broken organs and treatment of degenerative illness. ADSCs are believed to be promising supply of regenerative medicine, and EVs derived from ADSCs are believed to have comparable effects as well. Right here, we analysed lymphangiogenesis induced by EVs derived from ADSCs for therapy of chronic lymphedema. Solutions: EVs derived from ADSCs were isolated by CD314/NKG2D Proteins Storage & Stability ultracentrifugation. The effect of EVs to lymphatic endothelial cells (LECs) were analysed in proliferation assay, migration assay and tube formation assay. Gene expression analyses had been also performed by qRT-PCR. LECs were treated with PBS as control, VEGF-C(ten ng/ ml) and ADSC-EVs(100 g/ml) 1 time in every assay.Introduction: Extracellular vesicles (EV)-mimetic nanovesicles (NVs) includes various protein, mRNA and miRNA and is known to play an essential part in intercellular communication as a bio-nanoparticle with a diameter of 40 to one hundred nm. Recent studies have demonstrated the therapeutic possible of EVmimetic NVs within a variety of animal models for cardiovascular diseases and neuropathies. The aim of this study was to investigate effectiveness of embryonic stem cell (ESC)-derived EV-mimetic NVs in restoring erectile function in diabetic mice. Strategies: Di.