We hypothesized that the certain proteins containing EV released from stroke serum cultured MSCs could have an effect on the neurogenesis and angiogenesis of recipient cells. Procedures: EVs have been purified from conditioned media of MSC cultured with FBS (FBS-MSC) and MSC cultured with stroke serum (SS-MSC). These EVs have been characterized by nanoparticle tracking analysis. EV protein profiling in conditioned media was systematically compared via using LC-MS/MS-based label-free quantification. Real-time PCR was performed to identify the distinction inside the gene expression in each cell. The protein concentration in the EV was confirmed by ELISA. Results: A total of 1068 proteins had been identified from SS-MSC-EV and FBS-MSC-EV by means of LC-MS. In accordance with statistical evaluation, 22 proteins had been discovered to be far more than 2-fold (p 0.05) upregulated in SS-MSC_EV. ITGA5, CLU, and CTSB have been considerably elevated of SS-MSC gene expression levels in comparison with FBS-MSC. Amongst the candidate proteins, clusterin (CLU) was identified to be upregulated in EVs from SS-MSC when compared with those from FBS-MSC. α4β1 supplier Summary/Conclusion: These results suggest that SSMSC_EVs containing clusterin may possibly promote intercellular communication and influence neurogenesis and angiogenesis of recipient cells. Funding: This study was supported by a grant in the Korean Healthcare Technology R D Project, Ministry of Wellness Welfare (HI17C1256) and Standard Science Analysis Plan, the Ministry of Science, ICT and Future Preparing (2018M3A9H1023675).PT10.Adipose-derived Stem/Stromal Cell secretome, containing both soluble things and extracellular vesicles, exerts chondroprotective effects in vitro Chiara Giannasia, Stefania Niadaa, Sara Casatib and Anna BrinicaPT10.Proteomic analysis of extracellular vesicles from MSC cultured with stroke serum Yeon Hee Choa, Eun Hee Kima, Dong Hee Kimb, Ji Hee Sunga, Mi Jeong Oha, Eun Kyoung Shina and Oh Young BangaaIRCCS Istituto Ortopedico P2X3 Receptor Molecular Weight Galeazzi, Milano, Italy; bDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milano, Italy; cDepartment of Biomedical, Surgical and Dental Sciences, University of Milan. IRCCS Istituto Ortopedico Galeazzi, Milano, ItalySamsung Medical Center, Seoul, Republic of Korea; University, Seoul, Republic of KoreabSungkyunkwanIntroduction: Serum from stroke sufferers increases mesenchymal stem cells trophism towards the infarctedIntroduction: Up to now various clinical trials have shown the safety and efficacy on the intra-articular injection of Adipose-derived Mesenchymal Stem/ Stromal Cells (ASCs) in contrasting osteoarthritis.ISEV2019 ABSTRACT BOOKSince ASCs act predominantly by way of paracrine mechanisms, their secretome represents a promising cell-free option. Right here we identified anti-hypertrophic and anti-catabolic effects of ASC conditioned medium (ASC-CM) on TNF-stimulated human major articular chondrocytes (CHs). Procedures: CHs have been treated with 10 ng/mL TNF and/ or ASC-CM administered at a 1:five recipient:donor cell ratio. Cell viability was assessed up to day 9. The activity, expression and/or release of hypertrophy markers (MMP-13, Collagen X and Osteocalcin), catabolic mediators (MMP-3) and cartilage-protective elements were assessed up to day three by enzymatic assays, qRTPCR, Western Blot and multiplex immunoassays. Final results: ASC-CM blunted TNF-induced hypertrophy, reducing the enhanced levels of MMP-13 activity (-61), Osteocalcin (-37) and Collagen X (-18). In addition, also MMP-3 activity was diminished by -59.