To simulate drought tension, and transcriptomic analysis was applied to reveal the modifications of gene expression patterns in Amorpha fruticosa L. seedlings. Final results. Results showed that Amorpha fruticosa L. seedlings have been seriously affected by PEG-6000. As for the differently expressed genes (DEGs), the majority of them were up-regulated. The extra Go and KEGG analysis outcomes showed that DEGs were functionally enriched in cell wall, signal transduction and hormonal regulation associated pathways. DEGs like AfSOD, AfHSP, AfTGA, AfbZIP and AfGRX play roles in response to drought tension. Conclusion. In conclusion, Amorpha fruticosa L. seedlings had been sensitive to drought, which was diverse from Amorpha fruticosa L. tree, as well as the genes functions in drought pressure responses via ABA-independent pathways. The up-regulation of Salicylic acid signal associated DEGs (AfTGA and AfPR-1) indicated that Salicylic acid play a crucial function in response to drought pressure in Amorpha fruticosa L.Subjects Genomics, Plant Science Keywords Amorpha fruticosa L., Drought stress, Transcriptomic evaluation, Tolerance mechanismsSubmitted six July 2020 Accepted 9 February 2021 Published 22 March 2021 Corresponding author Qingjie Guan, [email protected] Academic editor Jacqueline Batley Extra von Hippel-Lindau (VHL) Synonyms Details and Declarations is often located on web page 12 DOI 10.7717/peerj.11044 Copyright 2021 Sun et al. Distributed under Creative Commons CC-BY 4.0 OPEN ACCESSBACKGROUNDAmorpha fruticosa L. is actually a deciduous shrub that is native to North America and has been introduced to China as an ornamental plant (Hou, 1982; Wang et al., 2002). AsHow to cite this article Sun X, Hu S, Wang X, Liu H, Zhou Y, Guan Q. 2021. De novo assembly of Amorpha fruticosa L. transcriptome in response to drought tension supplies insight into the tolerance mechanisms. PeerJ 9:e11044 http://doi.org/10.7717/peerj.a kind of urban greening and slope protection plant, Amorpha fruticosa L. has high ornamental value and is widely used within the construction of urban landscape and road slope protection. Moreover, Amorpha fruticosa L. also has medicinal worth, including cytotoxic rotenoid glycosides, TBK1 list antibacterial and cytotoxic phenolic metabolite in seeds (Wu et al., 2014; Muharini et al., 2017). Furthermore, Amorpha fruticosa L. leaf was a type of conventional Chinese medicine utilized for the therapy of fever, burns, pyogenic carbuncle and eczema (Wu et al., 2014; Hovanet et al., 2015). Amorpha fruticosa L. can tolerate dry soils, nevertheless it is most abundant along river banks and roads and also the edges of flooded forests, even is tolerant of occasional flooding (Kozuharova et al., 2017). The high tolerance of various habitat conditions and potent propagation capacity promotes the aggressive invasive behavior of Amorpha fruticosa L. outdoors of its native range (Kozuharova et al., 2017). Understanding the drought tolerance mechanism of Amorpha fruticosa L. is of wonderful significance for the study of plant tolerance. Drought anxiety is one of the most prevalent environmental aspects limiting plant development (Bray, 2007). Distinct plants adapt to drought tension in the atmosphere via distinctive mechanisms, but most plants could response to drought pressure by means of hormonal regulation, which include abscisic acid (ABA), cytokinin (CK), gibberellic acid (GA), auxin, and ethylene, and so forth, which regulate diverse processes and enable plant adaptation to drought anxiety (Wilkinson et al., 2012; Basu et al., 2016). Several genes associated with hormonal regulation have already been pro.