According to many gene markers and morphological comparisons suggest that so-called
Based on a number of gene markers and morphological comparisons recommend that so-called F. velutipes in East Asia, unlike the European winter mushroom F. velutipes, needs to be treated as a separate species, namely F. filiformis [25]. A equivalent trouble was reported for Jin’er, which was previously reported as Tremella mesenterica [26]. Bandoni R.J. studied the morphological capabilities of Jin’er and named it T. aurantialba [11]. Until 2015, Liu et al. investigated the phylogenetic relationship of Tremellomycetes by phylogenetic trees constructed by seven gene sequences, eventually naming them N. aurantialba [27]. Consequently, it’s necessary to further clarify the taxonomic status of N. aurantialba genetically from the population level. In current years, the genomes of some basidiomycetes have been obtained, including Agaricus bisporus [28], Auricularia heimuer [17], Coprinopsis cinerea [29], G. lucidum [30], Hericium erinaceus [21], Lentinula edodes [31], Naematelia encephala [32], Tremella fuciformis [33], and T. mesenterica [34]. The availability of these increased genome sequences has promoted study on gene diversity and the identification of genes involved within the biosynthesis of secondary metabolites through genome mining. Despite the fact that N. aurantialba has quite a few crucial characteristics, you can find only about 13 accessible nucleotide sequences for N. aurantialba in the National Center for Biotechnology Details (NCBI) database, the majority of which are utilized for phylogenetic analysis. Therefore, the present genetic sequence sources are usually not sufficient to reveal the pharmacological mechanism of N. aurantialba in the molecular level. As a result, within this study, we aimed to introduce the whole genome sequence of N. aurantialba NX-20 and to elucidate the its genome by means of comparison using the genomes of 18 basidiomycetes. We also aimed to investigate functional annotations (Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (KOG), Transporter Classification Database (TCDB), etc.) to predict the genes or gene clusters involved in the biosynthesis of Telomerase Inhibitor Molecular Weight polysaccharides as well as other secondary metabolites. 2. Components and Approaches two.1. Fungal Strains and Strain Culture The fruiting bodies of N. aurantialba have been Syk custom synthesis collected from Kunming, Yunnan Province, China (Figure 1). A single spore strain was obtained in the fruiting body by the spore ejection approach, and also the strain was identified as N. aurantialba, which we named N. aurantialba NX-20 [35]. At present, this strain has been preserved inside the China Basic Microbiological Culture Collection Center (CGMCC 18588). To receive adequate cell amounts for genomicJ. Fungi 2022, 8,three ofJ. Fungi 2022, eight,ejection strategy, along with the strain was identified as N. aurantialba, which we named N. au rantialba NX20 [35]. At present, this strain has been preserved in the China General Mi crobiological Culture Collection Center (CGMCC 18588). To obtain adequate cell amounts DNA extraction, N. extraction, N. aurantialba NX20 was inoculated into potato dextrose for genomic DNA aurantialba NX-20 was inoculated into potato dextrose broth medium and grown at 25 C with continuous shaking (200 rpm) for three d [35]. broth medium and grown at 25 with continuous shaking (200 rpm) for three d [35].3 ofFigure 1. Fruiting bodies of N. aurantialba. Figure 1. Fruiting bodies of N. aurantialba.2.2. Extraction of Genome DNA two.2. Extraction of Genome DNA After fermentation, the spore cells had been collected.