D analyzed by the established method. Each and every sample was determined in 3 instances. The average recoveries had been estimated by the formula (1):The precision on the process have been studied by determination of interday and intraday variances. The interday and intraday precisions were evaluated by measuring a typical mixture answer composed of 12 elements at three concentrations six times every day and twice every day over three consecutive days separately beneath the optimized MC3R Agonist Compound conditions. The relative standard deviation (RSD) was applied to estimate interday and intraday precisions. As the final results shown in Table three, RSD values were all 2.89 ponent Protopine Allocryptopine Coptisine Xanthotol Palmatine Dehydrocorydaline Glaucine Tetrahydropalmatine Tetrahydroberberine Imperatorin Corydaline IsoimperatorinLOD: Limit of detectionSample analysisThe validated analytical approach was successfully applied towards the simultaneous determination of PTE, ATP, CTE, XTL, PME, DCE, GCE, THP, TDE, IMP, CDE, ISO in 4 Tyk2 Inhibitor manufacturer dosage for ms of YZP containing 17 batches from the very same manufacturer o r di ffer en t ma nufa ctur er s. E a ch sa m p l e wa s determined in three instances. As shown in Table 6, the outcomes demonstrated that all batches of YZP wereLinear range (g/mL) 0.86 86 1.25 50 0.48 48 0.50 50 0.50 50 0.50 one hundred 0.49 98 2.60 104 1.60 64 0.63 125 0.98 98 1.05 105 LOD (g/mL) 0.17 0.13 0.12 0.10 0.13 0.13 0.12 0.10 0.16 0.15 0.10 0.Table 2: Calibration curves, linear ranges, LOD of 12 elements (n=3)Regression equation y=14705.94x+763.44 y=14193.25x+2291.63 y=49300.26×070.76 y=34988.36x+6743.02 y=59456.50x+3756.77 y=59233.72x+22068.13 y=33853.65x+13903.55 y=16706.45x+11343.23 y=15094.90x-7061.49 y=26669.44x+10516.27 y=14717.36×95.90 y=19685.95x+8654.52 r2 0.9999 0.9999 0.9998 0.9999 0.9999 0.9999 0.9999 0.9999 0.9981 0.9998 0.9999 0.Pharmacognosy Magazine | January-March 2015 | Vol 11 | IssueZhang, et al.: Quantitative Determination of Active Elements in Yuanhu Zhitong PrescriptionTable 3: Intraday and interday precisions of 12 components (n=6, ��SD)Component Original (g mL-[1]) 2.15 eight.60 86.00 1.25 five.00 50.00 1.20 4.80 48.00 1.25 5.00 50.00 1.25 5.00 50.00 2.50 ten.00 one hundred.00 two.45 9.80 98.00 2.60 10.40 104.00 1.60 6.40 64.00 3.13 12.50 125.00 2.45 9.80 98.00 two.51 ten.50 105.00 Precious Intraday Mean D (g mL Protopine two.07.01 eight.49.09 85.12 .21 1.16.01 4.59.05 48.81.72 1.15.01 4.41.10 45.65.73 1.27.02 four.92.04 49.31 .41 1.17.02 4.85.ten 49.71 .23 two.50.02 ten.02.07 99.78 .32 2.54.04 9.91.05 97.00 .34 two.51.04 9.92.06 103.11 .54 1.51.03 6.22.05 58.41.30 3.24.03 12.25.11 124.03 .82 two.38.05 9.45.10 97.09.38 2.52.05 10.23.15 103.89.-[1]Interday RSD ( ) 0.63 1.11 0.24 1.03 1.03 1.47 0.71 2.22 1.60 1.44 0.71 0.83 1.32 two.13 0.47 0.71 0.71 0.32 1.40 0.52 0.35 1.39 0.64 0.52 two.20 0.75 0.52 1.00 0.92 0.66 2.22 1.02 0.39 1.87 1.42 0.52 Imply D (g mL-[1]) two.06.02 8.47.ten 85.36.51 1.16.02 4.64.07 48.84.72 1.15.03 four.41.02 45.18.73 1.27.03 4.91.06 49.27.33 1.17.02 4.89.09 49.56.43 2.49.02 10.04.08 99.63.18 two.52.03 9.91.04 97.33.52 2.52.02 9.98.07 103.00.30 1.51.04 6.22.05 58.34.25 three.25.02 12.27.06 123.84.75 2.39.06 9.50.ten 97.08.62 two.50.05 ten.30.24 103.65.79 RSD ( ) 0.92 1.15 0.60 1.61 1.50 1.48 two.89 0.48 1.62 2.26 1.31 0.68 1.30 1.93 0.87 0.70 0.83 0.18 1.37 0.43 0.54 0.98 0.69 0.29 2.46 0.81 0.43 0.71 0.52 0.61 two.53 1.ten 0.64 1.92 two.28 0.)AllocryptopineCoptisineXanthotolPalmatineDehydrocorydalineGlaucineTetrahydropalmatineTetrahydroberberineImperatorinCorydalineIsoimperatorinSD: Regular deviation, RSD.