Spac was inserted into the plasmid pUB-Tet, plus a new replication-thermosensitive
Spac was inserted in to the plasmid pUB-Tet, in addition to a new replication-thermosensitive plasmid, pUB-MazF, was constructed (Fig. 1d).Functional Verification of pUB-MazF and pUB -EXThe replication of pUB-MazF was…