Product Name :
Streptavidin(iFluor594)
Applications:
ELISA,WB,Flow Cyt
Reactivity :
Biotin
Conjugate:
iFluor594
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Streptavidin(iFluor594) is coupled steptavidin with iFluor594. Streptavidin(iFluor594) is highly sensitive to binds biotinylated proteins in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry systems.{{34233-69-7} medchemexpress|{34233-69-7} Technical Information|{34233-69-7} Data Sheet|{34233-69-7} custom synthesis} | Conjugate: iFluor594(Ex: 590nm, Em: 617nm), 2 moles iFluor594 per mole streptavidin | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, protect from light | Background:Streptavidin is a 53 kDa protein isolated from Streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and contains 4 subunits.{{1421438-81-4} medchemexpress|{1421438-81-4} Purity & Documentation|{1421438-81-4} Formula|{1421438-81-4} supplier} Each subunit can bind one molecule of biotin.PMID:25905259 Streptavidin has strong binding properties to avidin (Kd=10-15). Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins.
Description2 :
Streptavidin(iFluor594) is coupled steptavidin with iFluor594. Streptavidin(iFluor594) is highly sensitive to binds biotinylated proteins in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry systems.
Immunogen:
Host :
Isotype:
Purity :
Buffer :
Storage :
Store at –20 °C, protect from light
Function:
WB: 1:10,000-1:50,000 ELISA: 1:10,000-1:50,000Flow Cyt: 1:200-1:2000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.