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Neuropeptide Y receptor Y1 polyclonal antibody
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Neuropeptide Y (NPY) and the closely related peptide YY (PYY) and pancreatic polypeptide (PP) are a family of 36-amino acid, tyrosine-rich peptides with amidated C-terminal ends that are known to regulate gastrointestinal and central nervous system functions including food intake and the inhibition of anxiety. Four functional NPY receptors (Y1/Y2/Y4/Y5) have been identified to date, with a fifth (y6) being a non-functional receptor in primates that is absent from the rat genome. Y1, Y2, and Y5 receptors preferentially bind NPY and PYY, while the Y4 receptor preferentially binds PP. All four receptors signal via coupling to Gi/o alpha subunits, inhibiting adenylyl cyclase activity, thereby decreasing intracellular cAMP levels. Y1 receptor deficient mice display a variety of physical and behavioral phenotypes including obesity, hyperinsulinemia, and increased territorial behavior. Western blot analysis: MW marker (1) and 20µg rat brain extract (2) probed with Neuropeptide Y Receptor (Y1) pAb at 5.1µg/ml. Membrane ELISA: Lewis rat heart membranes (5µg/well) were treated with 1μM concentrations of agonist (NPY) and probed with Neuropeptide Y Receptor (Y1) pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4). Western blot analysis: MW marker (1) and 20µg rat brain extract (2) probed with Neuropeptide Y Receptor (Y1) pAb at 5.1µg/ml. Membrane ELISA: Lewis rat heart membranes (5µg/well) were treated with 1μM concentrations of agonist (NPY) and probed with Neuropeptide Y Receptor (Y1) pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA.{{138777-25-0} web|{138777-25-0} Biological Activity|{138777-25-0} In Vitro|{138777-25-0} custom synthesis} Data from vehicle treated cells were taken as 100%.{{1977546-93-2} medchemexpress|{1977546-93-2} Technical Information|{1977546-93-2} In Vitro|{1977546-93-2} custom synthesis} Results are the mean ± SEM (n=4).PMID:30020711
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| Alternative Name NPY1R, NPYR | Application ELISA, WB | Application Notes Suitable for membrane-based ELISA.Predicted MW of ~44kDa. Higher MW species (~80kDa) also observed by Western blot which may reflects post-translational modification or dimerization of the receptor. | Crossreactivity Predicted species reactivity based on epitope sequence homology: horse, chimpanzee, rhesus macaque, guinea pig. | Formulation Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. | Host Rabbit | Immunogen Synthetic peptide corresponding to the sequence near the N-terminus of rat neuropeptide Y receptor Y1. | Species Reactivity Human, Mouse, Rat | UniProt ID P21555 | Unit of Measure (UM) µg