Ague ,Division of Physiology,Faculty of Medicine in Hradec Kralove,Charles University in Prague,Hradec Kralove,Czech Republic Get in touch with E-mail Address: josef.fontanalf.cuni.cz Introduction: Nonalcoholic fatty liver disease (NAFLD) is one of the most typical liver diseases in western countries,affecting of adult population. This situation can progress to much more serious liver diseases nonalcoholic Dehydroxymethylepoxyquinomicin biological activity steatohepatitis,cirrhosis or hepatocellular carcinoma . Incretin hormone glucagonlike peptide (GLP) exerts helpful effects on liver functions and liver metabolism,in particular in NAFLD condition. In our earlier experiment we described a diminishing effect of GLP analogue Liraglutide (LIRA) on an early phase of liver regeneration immediately after partial hepatectomy in rats. In our present perform we evaluated an effect of LIRA on cell viability and oxidative pressure parameters in primary cultures of rat hepatocytes isolated from lean and steatotic livers. Aims Solutions: Key cultures of hepatocytes were obtained from male Wistar rats fed a normal laboratory diet regime (STgroup, of energy from fat) or maybe a highfat diet (HFgroup, of power from fat) for weeks. Hepatocytes had been isolated by a twostep collagenase perfusion of rat liver,cell viability was . Just after the establishment of monolayers,hepatocytes were incubated in supplemented Williams’ E medium containing LIRA at concentrations of . nmoll for hours. Soon after this period the medium was collected for biochemical assays. We assessed cell viability (leakage of lactate dehydrogenase (LDH) and activity of cellular dehydrogenases WST assay),synthetic capacity of the hepatocytes (production of albumin determined by ELISA) and markers of oxidative stress (malondialdehyde concentration (MDA),and DCFDA assay). Outcomes: HFgroups vs. Recent research described a higher prevalence of vitamin D deficiency in sufferers with distinctive liver ailments and portal hypertension. Aims Strategies Aims: The authors propose to assess vitamin D deficiency in individuals with liver illness and its association with clinical and analytical parameters related to hepatic and phosphocalcium metabolism. Strategies: Retrospective analysis of clinical records of sufferers followed on Hepatology Clinic within a single center. A vitamin D level ngmL was thought of as deficiency,according PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26212255 to suggestions. FibroScan transient elastography was utilized to estimate liver stiffness. Final results: In this study were incorporated sufferers: men using a median age of years,of which sufferers had liver cirrhosis. The most popular etiologies had been VHB infection (alcohol ( and VHC infection Median vitamin D levels have been (IQR: ngmL. Vitamin D deficiency was detected in of total sample, in those with cirrhosis and in those without the need of cirrhosis. Patients with vitamin D deficiency had reduce calcium serum levels (p.) and enhanced hepatic elastography (p.). In multivariate analysis,applying logistic regression,vitamin D deficiency was independently related with liver cirrhosis (OR p.) along with the albumin levels were a protective element (OR p.). Conclusion: Vitamin D deficiency is quite prevalent in liver disease,even devoid of cirrhosis. This deficiency can lead to hypocalcemia,and need to be prevented. Our results suggest that this deficiency might be mainly connected with the severity of hepatic disease as reflected by the protective role of albumin levels and also the association with cirrhosis and enhanced hepatic elastography. Disclosure of Interest: None declaredContact E mail Address: nelzouki_yahoo Introductio.