Ences in the parameters among the therapies at p 0.05 according to
Ences within the parameters in between the remedies at p 0.05 as outlined by Tukey test.two.5. Data Visualization and Analysis–Relationship among Distinct Treatment options and Observed Parameters A heatmap with dendrograms was applied to analyze and visualize the information from the study (Figure 9). The dendrograms sort the remedies utilized in our study (control, 10-11 M IBA, 10-7 M IBA, Cd, Cd 10-9 M IBA, 10-9 M IBA), also because the parameters studied (development parameters, activity of antioxidant enzymes, concentration of H2 O2 and of nutrients) according to their similarity. The lateral dendrogram reveals a similarity amongst the handle plus the 10-9 M IBA treatment. Both the manage and the 10-9 M IBA therapy are grouped within a cluster collectively with all the 10-11 M IBA treatment. The heatmap shows that these treatments hadGNE-371 Purity & Documentation Plants 2021, ten,8 ofmostly positive or no effects on the parameters studied. Yet another cluster is formed by the treatments: Cd, Cd 10-9 M IBA, and 10-7 M IBA, which share frequent options. The coloring clearly shows the raise within the activity of antioxidant enzymes, reduce inside the growth parameters, and concentrations of nutrients in comparison with all the control. It can be noteworthy that there is a closer partnership between 10-7 M IBA and Cd 10-9 M IBA remedies than in between the Cd 10-9 M IBA and Cd treatment options.Figure 9. The heatmap analysis with the parameters studied within the treatment options: handle, 10-11 M IBA, 10-7 M IBA, Cd, Cd 10-9 M IBA, 10-9 M IBA. Precisely the same colour indicates a similar value on the parameter. Every single worth of each parameter is expressed as a percentage of its corresponding control. PR elongation-elongation of principal root; PR branching-branching of principal root; LR number-number of lateral roots; fresh weight-root fresh weight; dry weight-root dry weight.Whereas the upper dendrogram in the heatmap indicates the relationships amongst parameters (Figure 9), the GS-626510 manufacturer correlation matrix heatmap presents an insight into the correlation amongst them (Figure 10). The powerful good correlation was detected in between the activity of antioxidant enzymes as well as the concentration of H2 O2 (Figure 10). On the other hand, these parameters correlate negatively using the development parameters and also the concentration of nutrients. A strong correlation was detected amongst the concentrations of nutrients (using the exception of Mg) and biomass (fresh weight and dry weight). The highest Pearson coefficient values (range from 0.8 to 0.96) was detected amongst the biomass (fresh weight and dry weight) and also the concentrations of P, S, Ca, Cu, K, Fe, and N. The concentration of Cd correlates positively together with the activity of antioxidant enzymes plus the concentration H2 O2 . Nonetheless, the concentration of Cd had powerful damaging correlation with nutrients, using the lowest Pearson coefficient values (range from -0.eight to -0.97) amongst the concentration of Cd as well as the concentrations of Fe, Mn, N, K, S, and P.Plants 2021, 10,9 ofFigure 10. The correlation matrix heatmap shows the values from the Pearson correlation coefficient for all studied parameters, the optimistic values are in red, adverse are in blue. The coefficient ranges from -1 (perfect damaging linear relationship) to 1 (best positive linear partnership), even though the value 0 indicates that there’s no relationship amongst studied variables. The parameters analyzed had been the elongation of key root (PR elongation), PR branching, variety of lateral roots (LR quantity), fresh and dry weight, the activity of enzymes APX, CAT, S.