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Preeclampsia (PE) affects 5 to 8 of pregnancies and has severe consequences for both the mother and fetus [1]. The pathogenesis of PE remains elusive. Long-chain 3-hydroxyacylCoA dehydrogenase (LCHAD) deficiency has been found associated with complications of pregnancy such as hemolysis, elevated liver enzymes, and low platelet syndrome (HELLP) and acute fatty liver of pregnancy [2]. We and other researchers found decreased LCHAD expression in some cases of PE without LCHAD deficiency and the expression of LCHAD significantly differed between early-onset severe PE and late-onset PE [5,6].Baicalein The heterogeneity of PE has been gradually realized by study of different etiological factors between early- and late-onset PE [7,8]. However, the role of long-chain fatty acid-oxidation (FAO)disorders in different pathogenesis of PE is unclear and deserves further study.Dispase The last three steps of FAO are catalyzed by the trifunctional protein (MTP); LCHAD is located on the a-subunit of MTP [9].PMID:23376608 LCHAD deficiency leads to long-chain FAO disorders. However, some PE patients without LCHAD deficiency also show longchain FAO disorders. Maternal plasma from PE patients could result in lipid droplet accumulation in cultured human umbilical vein endothelial cells and cause a significant decrease in mitochondrial dehydrogenase activity [10]. In animal experiments, we found significant pathological changes in placentas of Nw-nitro-L-arginine-methyl ester (L-NAME; L-NA)-established PE-like mice in early and mid-gestational stages as well as decreased LCHAD expression [11]. An in vitro cell culture study of placental trophoblasts showed that LCHAD mRNA and protein expression significantly decreased in early-onset severePLOS ONE | www.plosone.orgFatty Acid Oxidation in Different Preeclampsia-Like ModelsFigure 1. Mean arterial blood pressure throughout pregnancy in Pre (A) and Mid subgroups (B) and 24-hr urinary protein levels (C). The arrow indicates time of injection except for the b2GPI group. #P,0.05 compared with control. *p,0.05 compared with control and ApoC3+NS. `p,0.05 compared with control and other experiment groups. {P,0.05, compared with corresponding Mid-gestation group. Data are mean6SD, n = 10. Pre, pre-implantation. Mid, Mid-gestation. doi:10.1371/journal.pone.0109554.gPE and HELLP syndrome as compared with late-onset severe PE [12]. These findings indicate that disorders of long-chain FAO induced by decreased LCHAD.