The reaction mixtures containing 25 to 500 nM PARP1 were preincubated at space temperature for 5 min, as indicated in the determine legend
ted with 20 ng/ml EGF in starvation medium (DMEM supplemented with 0.1% serum) for 30 min at 37. Cells were washed with ice-cold 1�PBS and harvested in ice-cold RIPA Corticotropin-releasing…