The infectious types were selected and purified through PureYield Plasmid Maxiprep Method (Promega), and sequenced to confirm that the entire env gene was entirely inserted
That's why, the BsiWI website was inserted at placement 6202 in HXB2, in a conserved sequence (ninety% conserved in the CRF01_AE and subtype C vpu sequences) of the cytoplasmic area…