Experimentswww.jove30. Block in 5 nonfat milk in TBS-T 0.1 O/N. Blot
Experimentswww.jove30. Block in five nonfat milk in TBS-T 0.1 O/N. Blot both membranes with anti-CFTR mouse monoclonal antibody CFF596 in addition to a goat antimouse HRP-conjugated secondary antibody. 31. Perform…