This treatment was adopted by therapy with propylene oxide (15 min), a one:1 Epon-acetone combine (2 h) and a few alterations in pure epon (2 times for three h and overnight)
Astrocyte samples have been processed utilizing Mobile Lysis and Protein Extraction kits (Sigma, United states). A modest amount of protein (thirty mg) was divided with sodium dodecyl sulfate-polyacrylamide gel electrophoresis…