Cells of strains to be tested had been collected from clean YPD plates, washed two times with PBS and recovered by minimal velocity centrifugation then, 106 yeast cells (in 250 L) ended up inoculated into the lateral tail vein of BALB/c mice and the mortality was adopted throughout 15 times
Yeast strains utilised in this operate are summarised in Table 1. Except if normally said, the mutant strains employed here are all homozygous null mutants (deletion of both alleles) in…