Cells had been co-transfected with the earlier mentioned luciferase reporter plasmids and a TK promoter/Renilla luciferase interior manage plasmid (pRLTK, Promega) at a 50:one ratio utilizing lipofectamine

Soon after 24 hours, mobile lysates were gathered and luciferase activity was measured using the Dual-Luciferase Reporter Assay Kit (Promega) and a TD-twenty/20 luminometer (Turner Patterns). For every single sample,…