For BrdU+/NeuN+ cells had been deemed new experienced neurons, BrdU+/S100B+ cells had been regarded as new mature astrocytes, and EdU+/DCX+ cells were deemed new immature neurons

Immunofluorecent or light-weight-level immunohistochemistry adopted common protocols . For fluorescent labeling, four% paraformaldehyde fixed 40 mm thick free of charge-floating sections had been rinsed 3 times in PBS and then…